Whether someone on your team needs to do a "quick" application vulnerability analysis, or you are a security researcher that makes your living doing this type of work, here are two open source tools that might help.
Both of these have been released by Cisco Talos as open source efforts, and their own researchers have found them very helpful. Just be aware that they do not come with support.
"The Mutiny Fuzzing Framework is a network fuzzer that operates by replaying network traffic through a mutational fuzzer. The goal is to begin network fuzzing as quickly as possible, at the expense of being thorough."
Download Mutiny Fuzzing Framework:
https://github.com/Cisco-Talos/mutiny-fuzzer
Decept Proxy is a multi-purpose network proxy, with a nice combination of capabilities:
The Decept Proxy can be found on GitHub here:
https://github.com/Cisco-Talos/Decept
Says Cisco AIG researcher James Spadaro, "The Mutiny Fuzzing Framework and Decept Proxy have been an effective toolset for Cisco in evaluating a variety of network applications and devices. This includes a number of Cisco devices which have been hardened based on bugs and vulnerabilities that were identified by network fuzzing."
On top of this success, the price is right!